Sentence examples for method for wnv from inspiring English sources

Results suggest that VecTest may be an efficient method for WNV detection in field-collected, dead American Crows, although confirmation of results and further investigation are warranted.

While new molecular WNV surveillance tests have been developed in recent years, many veterinary diagnostic laboratories still use immunohistochemistry as a primary method for WNV surveillance in AMCRs.

This interest is based on recent experience indicating that surveillance of bird deaths, especially of crows and other members of the family Corvidae, is a sensitive method for detecting WNV activity in a region (2 – 5 ).

Detection of IgM in serum or cerebrospinal fluid (CSF) is the preferred method for diagnosing WNV infection; however, because of cross-reactivity between flaviviruses, positive results should be confirmed by virus neutralization assay.

The results of our study also confirm observations by others (14 ) that surveillance of dead birds is a sensitive method for detecting early WNV activity.

RT-PCR methods for detection of WNV RNA in tissues were according to those of Lanciotti et al. (14 ), except for use of the Viral RNA Minikit (QIAGEN, Valencia, CA, USA) for RNA extraction and the Bio-Rad Icycler IQ Real-time Detection System (Bio-Rad, Hercules, CA, USA) for cDNA amplification.

Nineteen samples were positive for WNV with a faint-to-bold WNV line; one sample was negative for WNV.

These serum specimens were collected from febrile volunteers in Kathmandu and Bharatpur and tested for WNV using a nested RT-PCR adapted from methods previously reported [ 7, 8].

The results of the equine IgM-capture ELISA for WNV antibody indicate that this is a simple and efficient method for detecting antibody at about the same time that neutralizing antibody can be detected.

These healthy individuals tested positive for WNV infection and were therefore classified as subclinical seroconverters.

Serology for WNV and dengue virus was assessed by PRNT as described previously [31].