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Hence, it has been postulated to be a promising therapeutic method for viral diseases such as HIV, hepatitis B and the Ebola virus4,5,6.
A novel LUX (Light Upon eXtension) primer-based real-time PCR assay was developed and evaluated in this study, which was designed to provide a cost-effective, specific and highly sensitive method for viral load determination of hepatitis B virus (HBV).
The Virochip microarray was also analyzed using Z-score analysis and E-Predict, an automated method for viral species identification in microarrays [7], [35].
This method has also been proposed for HIV to try to standardize viral classification [38] confirming the interest of this method for viral classification.
DV and AL designed and performed the quantitative PCR method for viral load measurement.
We have presented VGA, an accurate method for viral population assembly from ultra-deep sequencing data.
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The use of simplified methods for viral load determination could greatly increase access to treatment monitoring of HIV patients in resource-limited countries.
The greater sensitivity of PCR methods for viral diagnostics allows much more reliable incidence estimates, particularly for norovirus and sapovirus.
Results of conventional methods for viral isolation, routinely performed (in case of respiratory disorder), were transmitted weekly to the clinicians.
Methods for viral growth, genomic RNA extraction, and amplification of viral sequences by reverse transcription polymerase chain reaction (RT-PCR) have been previously described (16 ).
Using the most sensitive methods for viral detection, we were able to identify that 22% of our patients had viruses in their airways.
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