Methods for uniquely identifying and quantifying phenotypic effects of mutant alleles in complex mixtures enable the parallel analysis of hundreds to thousands of genotypes.
We provide a detailed description of the methods for extracting uniquely mappable reads for snRNA paralogs from RNA-seq data, including command line instructions.
This trans-splicing method is uniquely suitable for protein C-terminal labeling, in contrast to a previously reported trans-splicing method using the S1 split-intein for protein N-terminal labeling [26], [29].
With these advantages, the method is uniquely suited for initial and exploratory analyses of diverse or poorly understood communities.
Together, these findings show that this method is uniquely suitable for elucidating the molecular mechanisms coupling transmembrane signaling to the assembly of complex AChR-rich postsynaptic membrane in vitro.
For all sulfamates examined, oxidation occurs with high selectivity at the γ-carbon, affording a uniquely predictable method for C-H bond halogenation.
Consequently, CPA offers a uniquely powerful method for investigating neuronal and O2-based signals simultaneously in behaving animals.
However, for a few targets, it provides a uniquely sensitive method for the detection/quantitation of trace components from small, but very complex, samples.
