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We describe the development and validation of a new quantitative real time PCR (qrt-PCR) method for the enumeration of the toxic benthic dinoflagellate Ostreopsis cf. ovata in marine environment.
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In 2004, the CellSearch system was introduced as the first and only FDA cleared method designed for the enumeration of circulating tumor cells in 7.5 mL of blood.
The culture media used are those recommended by Standard Methods [ 26] and also correspond to the official Mexican methods [ 46] for the enumeration of TC and FC in water samples.
This method is an alternative method to flow cytometry for the enumeration of peripheral CD4 lymphocyte in resource-limited settings.
This study was undertaken to evaluate modified qPCR methods as infectivity assays for the enumeration of infectious MS2 coliphage.
In addition, quantitative real-time PCR methods have been established for the enumeration of toxic Microcystis in environmental samples [ 86, 87].
Therefore, our results demonstrate a powerful method for hit explosion, the enumeration of compounds around scaffolds from a HTS experiment.
The results showed that this method can be used as a rapid technique for the enumeration of these bacteria in cheese samples.
Secondly, the checkerboard DNA-DNA hybridization technique has its strengths and limitations; the main advantage is that the method permits enumeration of large numbers of species in large numbers of samples and hence, considered as a useful tool for the enumeration of bacterial species in complex microbial systems [ 16].
Figure 1 Gating strategy for the enumeration of blood DC.
Violet red bile glucose (VRBG) agar is designed for the enumeration of Enterobacteriaceae.
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