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Additionally, there is no standardized method for susceptibility testing recommended by NCCLS for these microorganisms.
A standardized method for susceptibility testing of M. ulcerans is not currently available (25 ).
Selection of the most appropriate method for susceptibility testing can be problematic as the low percentages of CA and EA Etest demonstrated for some Candida species.
A common method for susceptibility testing of colistin has been the disk diffusion method that uses 10 micrograms of colistin sulfate disk (Oxoid, Basingstoke, Hants, England).
Because absorbance-based instruments can be found in most laboratories, we evaluated the applicability of the AB method for susceptibility testing of S. aureus biofilms using absorbance.
In a multicenter study, Espinel-Ingroff et al. evaluated agar diffusion method for susceptibility testing of filamentous fungi on plain Muller-Hinton agar medium [ 47].
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Conventional methods for susceptibility testing require several months before results can be reported.
There is a need for standardisation of national laboratory methods for susceptibility testing and for improving the surveillance of antimicrobial resistance to enable rapid revision of treatment guidelines when necessary.
The methods for susceptibility testing used to choose the appropriate drug are ADDM qualitative test and quantitative determinations by means of micro-dilution to determine the minimum inhibitory concentration (MIC) [ 3, 2].
The secondary method used for susceptibility testing was the disk diffusion method according to Kirby-Bauer [ 14].
Most laboratories used variants of Stokes' disc method (5 ) for susceptibility tests in the period reviewed, but a minority used breakpoint tests.
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