Exact(1)
Seroneutralization is described as the reference method for specificity (no cross-reaction with other phleboviruses) (7 ), but the need for cell culture makes it unsuitable for screening large numbers of serum samples (8 ).
Similar(59)
The presented structure-based approach is generalizable to other protease enzymes with known or modeled structures, and complements existing experimental methods for specificity determination.
Methods for specificity determinant prediction can be classified as those that require the subtypes to be known a priori (Capra and Singh, 2008; Hannenhalli and Russell, 2000; Kalinina et al., 2004; Mirny and Gelfand, 2002; Pirovano et al., 2006) and those that do not (Del Sol Mesa et al., 2003; Donald and Shakhnovich, 2005; Pei et al., 2006).
A new method for DNA specificity detection with high discrimination performance is established based on a novel silver nanoparticle coupled surface plasmon-coupled emission strategy (AgNPs coupled SPCE).
We will then go on to describe methods we have developed for assessing and quantifying patterns of immunogold labeling and finally describe a method for assessing specificity.
We demonstrate a simple and inexpensive method for high specificity and attomolar sensitivity detection of active BoNT/B in both PBS and honey through the dual binding of antibodies and monovalent or multivalent SL-DNA conjugates to toxins.
Previous studies by our group demonstrated that multimeric presentation of peptides on lysine cores is an effective method for retaining specificity and affinity of peptides selected from pIII displayed libraries.
Some of the methods proposed for specificity determinant prediction require the subtypes to be specified (Capra and Singh, 2008; Hannenhalli and Russell, 2000; Kalinina et al., 2004; Mirny and Gelfand, 2002; Pirovano et al., 2006), while others (Del Sol Mesa et al., 2003; Donald and Shakhnovich, 2005; Pei et al., 2006) do not.
The method validation assays were carried out according to the currently accepted US Food and Drug Administration FDAA) bioanalytical method validation guidance for specificity, linearity, sensitivity, precision, accuracy and recovery.
The method was validated for specificity, linearity, precision, accuracy and robustness.
It is also noteworthy that the detection and quantitation limits of this method for ruminant DNA are the lowest reported limits to date in crude heparin [ 10, 14] The analytical method was validated for specificity, linearity, accuracy, precision (repeatability and intermediate precision), and quantitation limit.
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