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Currently, the most common method for smear layer removal is the sequential use of ethylene diamine tetraacetic acid (EDTA) and sodium hypochlorite (NaOCl) solutions [ 2, 3].
For the current analysis, we included only patients without a positive sputum smear examination by the Ziehl-Neelsen technique, which was the standard method for smear examination at the time of the study.
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The PCR technique performance was compared with conventional routine diagnostic methods for smear negative patients.
Enhanced diagnostic methods for smear-negative tuberculosis are urgently needed.
In addition, the exceptionally high early mortality among TB patients (42% at two months) emphasizes the urgent need for diagnostic methods for smear-negative TB which are more accurate than clinical criteria yet equally rapid and affordable in low-income settings with a high incidence of HIV and TB.
The results reinforce the urgent need for novel diagnostic methods, both for smear positive and negative TB, that are highly sensitive, accessible and point of care, in order to reduce mean delay times.
Makkapati presented an improved wavelet-based image auto-focusing method for blood smears in microscope [8].
Sputum smear microscopy is the standard diagnostic method for detection of smear positive pulmonary tuberculosis (TB).
Sputum smear microscopy is the standard diagnostic method for detection of smear positive pulmonary TB in the DOTS strategy.
If a urethral discharge is present and can be adequately sampled without placing the loop, spatula or swab inside the meatus, this would be the recommended method for preparing a smear as it is likely to be preferred by the patient [ 37].
A dry 'paper smear' method for specimen collection and a simple single tube protocol was employed for PCR detection of HPV infection.
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CEO of Professional Science Editing for Scientists @ prosciediting.com