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We developed a surface plasmon resonance (SPR) method for serum transferrin assay that may aid standardization.
The system was used to analyze albumin in serum and urine samples and the results agreed well with the photometric bromocresal green method for serum sample and an immunoturbidimetric assay for urine sample (P > 0.05).
Currently, a standard method for serum exosome isolation is differential ultracentrifugation, but a search for alternative, less time-consuming and labour extensive exosomal isolation method for use in clinical settings is ongoing.
The traditional standardization method for serum creatinine was used.
We used the traditional standardisation method for serum creatinine.
Technical problems include selecting the best proteomics method for serum biomarker discovery [ 5].
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The variation in the results from different clinical laboratories testing the same patient sample using classical methods for serum transferrin determination can affect interpretation.
Given the rich lipid character of serum, lipidomic methods including lipid mediator profiling, DFI MS/MS and general lipidomics methods such as TLC/GC-FID-MS appear to be the most suitable methods for serum characterization – both in terms of their breadth of coverage and their amenability to quantification.
Robust methods for serum collection and processing were not described and this could have explained the lack of demonstrated association.
Finally, microextraction by packed sorbent was assessed as a suitable tool in forensic and clinical methods for serum sample preparations.
The authors report high correlation between GXM concentrations measured by the 2 methods for serum (0.93), for urine (0.94), and for plasma (0.94).
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