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The agreement between both techniques was assessed using the Bland and Altmann method for repeated measurements.
Diet and milking frequency had no effect on the TNF-α response ex vivo or CD14 expression level on monocytes, but cows fed diet H had significantly higher numbers of CD14+ monocytes than cows fed diet L. The WBA with LPS was a fast reliable method for repeated measurements of TNF-α responsiveness in cattle.
Second, the limits of agreement analysis was changed to Bland-Altman method for repeated data.
Linear mixed-model method for repeated measures to test for between-group differences with baseline values as covariates.
The missing data were imputed using a method for repeated measures with dropouts (Little and Rubin, 2002).
This underlines the concept of EBC as a non-invasive method and the usefulness of this method for repeated sampling.
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The primary outcomes (Aim 1a) are unlikely to require substantial attention to attrition, since measurement occurs shortly after randomization, and will not require methods for repeated measures.
Trial sequential analysis is an approach that combines conventional meta-analysis methodology with meta-analytic sample size considerations as well as previously established methods for repeated significance testing on accruing data in randomized trials [ 51].
The development of sensitive methods for repeated and noninvasive measurement of tumour biological activity during treatment needs to be attempted in order to optimise and individualise the treatment of childhood solid tumours.
In addition, this method allows for repeated and varied analyses to be carried out on the cells while they remain in a natural environment (i.e. whole blood).
Furthermore, this method allows for repeated measurements with minimal risk to the patient.
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