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Due to the relatively few VNTR-loci included and that there were only 33 isolates available for analysis in this study, sequencing was selected as a method for repeat number verification.
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Diet and milking frequency had no effect on the TNF-α response ex vivo or CD14 expression level on monocytes, but cows fed diet H had significantly higher numbers of CD14+ monocytes than cows fed diet L. The WBA with LPS was a fast reliable method for repeated measurements of TNF-α responsiveness in cattle.
The agreement between both techniques was assessed using the Bland and Altmann method for repeated measurements.
The p values of all main effects and interactions were corrected using the Greenhouse-Geisser method for repeated-measures effects.
Second, the limits of agreement analysis was changed to Bland-Altman method for repeated data.
This underlines the concept of EBC as a non-invasive method and the usefulness of this method for repeated sampling.
Linear mixed-model method for repeated measures to test for between-group differences with baseline values as covariates.
The missing data were imputed using a method for repeated measures with dropouts (Little and Rubin, 2002).
To compare differences between measurement times, analysis of variance (ANOVA, Holm-Sidak Method) for repeated measures was performed with SigmaStat software (SPSS Science, Chicago, USA).
Our analyses examined the responsiveness of the PVF and MA methods using a mixed-model method for repeated measures with time as fixed effect and subject as random effect, and provided fixed effect estimates by restricted likelihood modeling.
Ductal lavage (DL) is a novel method for repeated minimally invasive sampling of breast ductal fluid, allowing the safe collection of cells sufficient for cytological diagnosis and providing a source of cellular and free DNA for molecular analyses [ 5].
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