This work presents a new method for relative sizing between both components based on simple modelling of both polarisation curves.
In this paper, a new method for relative localisation, 'visiodometry', which does not rely on a kinematic model, is proposed.
A syndrome-based method for relative research has been conducted on the efficacy of TCDs of both cold and hot natures.
This communication highlight detection of two major chemical constituents, pk-I and pk-II for development and validation of an assay method for relative estimation of both these marker compounds in P. kurroa collected from seven different geographical ecozones of North Western Himalayas.
The Cq values were normalised to sample quantities using the standard curve method for relative quantification.
Data was analyzed using the Comparative Ct method (ΔΔCt) method for Relative Quantification (RQ).
The resulting data were analyzed with the comparative CT method for relative gene expression quantification against house keeping gene GAPDH.
We used the ΔΔCT method for relative quantification as the PCR efficiencies between the target(s) and reference gene(s) were relatively equivalent.
The comparative Ct method for relative quantification was used, whereby all threshold cycles (Ct) are first normalized to the expression of an endogenous control (18s rRNA).
Quantitation of pmp expression was achieved using the ABI 7000 SDS (Applied Biosystems), SYBR Green chemistry, and the standard curve method for relative quantitation, using reagents and thermocycling as previously described [17].
The primers and probes used are listed in Table 3 and the comparative CT method for relative quantitation (2−ΔΔCT) was used with rabbit β-actin as the endogenous housekeeping gene [6], [44].
