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Objective: To develop a simple and reliable method for quantitating plasma glutamate concentration and apply this method to monitor systemic glutamate levels during coronary artery bypass graft (CABG) surgery, a procedure associated with neurologic deficits.
After establishing a method for quantitating T cell cytolytic activity within tumors, we addressed whether cytolytic activity of tumor-infiltrating T cells increased concurrently with phenotypic maturation, and whether the kinetics of killing varied between CTL within tumors and their counterparts in peripheral lymphoid organs.
To evaluate Aβ neurotoxicity properly, it is important to employ an appropriate method for quantitating cell viability.
Recently, using an ultrasensitive method for quantitating methods, ctDNA was detected in 100% of patients with stage II IV NSCLC and in 50% of patients with stage I, with 96% specificity for mutant allele fractions down to ~0.02% [ 88].
The preferred method for quantitating bulky PAH adducts in occupationally exposed individuals has been P postlabeling; however, this technique is regarded by many to lack specificity, and has been largely supplanted by more accurate mass spectrometry methods that have the ability to measure the lower levels of binding observed in the general public (Angerer et al., 1997a; Himmelstein et al., 2009).
However, the use of the dot blot method for quantitating multiple different constructs (for example containing different truncations of a single protein when annotated domain boundary information is absent) could be very useful and the pre-screen and dot blot combination is useful for optimising culture conditions for a single protein.
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There are a variety of methods for quantitating viruses.
DOI: http://dx.doi.org/10.7554/eLife.02040.011 10.7554/eLiFigure40.012 figure 2—figure supplement 3. Comparison of image analysis methods for quantitating cdr2p fluorescence in the nodes.
In the long term, other spatially and temporally resolved methods for quantitating gene expression may emerge (reviewed by Crosetto et al., 2015).
To overcome these challenges, we employed the Amnis ImageStreamX, an image cytometer, as a novel method for both quantitating exosomes and measuring uptake by recipient bladder cancer cells, thus overcoming the limitations of the current tools.
These methods are especially important for quantitating data in the complex anatomical structures of the human body.
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