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In the past few years, researchers have also proved that cryo-EM is more than just a fallback method for proteins that won't crystallize.
As you know, it doesn't occur; but for reasons which I cannot now quite reconstruct, I wanted to see the patterns of protein synthesis right after fertilisation and, being lazy, used a continuous methionine label [a tagging method for proteins].
Based on this reaction, a new quantitative determination method for proteins has been developed.
Consequently, the concept becomes an electrochemical sensing method for proteins based on the protein peptide interaction.
Precipitation of proteins is considered to be an effective purification method for proteins and has proven its potential to replace costly chromatography processes.
The challenge is to calibrate (i.e., parametrize) this method for proteins.
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Jpn 62, 524 531 (1989)The early application of the 'thioester ligation' method for protein synthesis.
Hwang H., Petrey D., Honig B. A Hybrid Method for Protein-protein Interface Prediction Protein.
PLoS One.2016 Dec9 11(12):e0167617. Hwang H, Petrey D, Honig B. A hybrid method for protein-protein interface prediction.
FRAGFOLD is a state-of-the-art de novo fragment-assembly method for protein structure prediction43, 44, 72, 73.
The proposed technique provides a simple and robust method for protein patterning.
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