Sentence examples for method for phosphatase from inspiring English sources

We present improvements to the method for phosphatase zymography in rhizobox studies, involving (1) a systematic evaluation of 4-methylumbelliferone (4-MU -based calibration functions in relation to image exposure time and (2) the development of advanced image analysis tools for lateral and longitudinal distributions of phosphatase activity along barley roots (Hordeum vulgare L., cv Optic).

Type I collagen was also shown to inhibit expression of the enterocytic marker alkaline phosphatase (Matsumoto et al, 1990) in Caco-2, Colony 29 and HRA-19 cells using an enzymatic method for alkaline phosphatase assay.

In this work, we designed highly sensitive and selective luminescent detection method for alkaline phosphatase using bovine serum albumin functionalized gold nanoclusters (BSA-AuNCs) as the nanosensor probe and pyridoxal phosphate as the substrate of alkaline phosphatase.

Fixed sections were subsequently stained with haematoxylin and eosin (H&E) or by immunoperoxidase method for placental alkaline phosphatase (ALPP or PLAP) (Giwercman et al, 1991), in order to obtain the closest approximation of the histological content of the samples.

Herein is reported a method for the sensitization of protein tyrosine phosphatases (PTPs) to small-molecule inhibition.

We used this method for high-throughput identification of protein phosphatases involved in lipid droplet metabolism.

Based on a modified p-nitrophenyl phosphate method ([29], phosphatase activity was measured by dephosphorylation of 0.1 mL of 150 mM p-nitrophenyl phosphate in 1.5-mL Eppendorf tube, which contained 0.4 mL buffer (200 mM Na-acetate buffer, pH 5.2 for acid phosphatase or 200 mM Na-borate buffer, pH 8.2 for alkaline phosphatase), 0.4 mL deionized water and 0.1 mL liquid culture supernatant.

Calf intestinal phosphatase (CIP) was used as a control for phosphatase assays.

Calf intestine alkaline phosphatase (CIAP, Takara) was used for phosphatase treatment.

Using the aforementioned method, one phosphatase cassette did not yield transformants (Δ pph-9; NCU000434) and viable ascospores could not be isolated for four phosphatase mutants: Δ ppp-1 (NCU00043); Δ cna-1 (NCU03804); Δ pph-1 (NCU06630); and Δ div-12 (NCU02496).

For phosphatase treatment, cells were suspended in phosphatase buffer and lysed by mild sonication.