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In this study, a novel 3D silk fibroin biphasic scaffold for integrated IVD tissue engineering was fabricated by sequentially using the paraffin sphere-leaching method for annulus fibrosus (AF) phase and the phase separation method for nucleus pulposus (NP) phase.
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Therefore, in this paper, we present a proposed framework that consists of several methods that integrates together for nucleus segmentation and cytoplasm extraction.
MSL, JHC and YYL are inventors on a patent submission entitled: A method for Protein Translocating into Nucleus Using A Specific Peptide Sequence for.
Moreover, no impairment of viability was seen at 4 and 24 h of incubation of SWCNT with primary human macrophages, using the Trypan blue assay and the Hoechst 33342 staining method for visualization of cell nuclei (data not shown).
In contrast to previous works in automating the evaluation of cognitive impairment through speech analysis that relied on manual transcripts of audio recording, our system uses a novel method for automatically detecting syllable nuclei in order to measure prosodic features without the need for a transcription.
The results show that the proposed method is able to yield 92% accuracy for nucleus segmentation and 78% for cytoplasm segmentation.
On the other hand, to facilitate the detection of the interaction between nuclear proteins, we have adapted our method for detecting interactions inside the nucleus of eukaryotic cells by adding nuclear targeting sequences to the muNS-derived inclusion-forming proteins.
We present a multistage scanning and registration method for data of the subthalamic nucleus (STN) obtained using ultrahigh 7 T MRI on four human postmortem brain samples.
As far as we know, this is a novel rapid and high-accuracy method for determining the number of nuclei per spores in AMF.
Double-labelling immunohistochemistry for MGMT and a cocktail of non-tumourous elements provides a "one look" method for determining whether tumour cell nuclei are MGMT-positive or MGMT-negative.
We used a novel high-accuracy method for determining the number of nuclei in live spores and found that in 166 live spores of G. diaphanum, the number of nuclei per spore ranged from 20 to 748 for diameters of 33 to 109 μm, respectively.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com