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In the present study, fast and robust methods for long range RT-PCR amplification and subsequent next generation sequencing (NGS) were developed and validated on nine Type 1 and nine Type 2 PRRSV viruses.
Method - The next method is for long range shooters.
All simulations were carried out with periodic boundary conditions, using the particle mesh Ewald (PME) method [43] for long range electrostatic forces.
In this chapter, we describe our recently developed pseudobond Q-Chem Amber interface, which employs a combined electrostatic–mechanical embedding scheme with periodic boundary condition and the particle mesh Ewald method for long-range electrostatics interactions.
To study substrate dynamics, we perform MD simulations using an isobaric and isothermal ensemble (NPT) in explicit water while employing the particle-mesh Ewald method for long-range electrostatics.
After the position-restraint run, an unconstrained dynamics simulation was performed for 50 200 ns at a temperature of 300 K as an isothermal isobaric (NPT) ensemble under periodic boundary conditions using the PME (particle mesh Ewald) method for long-range electrostatics.
Combining the newly developed nonlinear model predictive static programming technique with null range direction concept, a novel explicit energy-insensitive guidance design method is presented in this paper for long range flight vehicles, which leads to a closed form solution of the necessary guidance command update.
The particle mesh Ewald (PME) method [ 20] was used with a direct-space non-bonded cutoff of 8 Å to account for long range interactions.
(h>1/2 allows for long range time dependence).
Excellent for long range damage.
these are great for long range shots.
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