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The results indicated that solid-state fermentation (SSF) using barley as the substrate is a suitable method for inoculation.
In this study, we established a laboratory unimpaired root dip-inoculation method for inoculation and found that it accurately reflected the progress of a natural infection of cotton by V. dahliae V592 (Figure 2A).
As the main objective for this research is to identify rice varieties that exhibit resistance or moderate resistance to R. solani, therefore it is important to select a quick, efficient, and consistent method for inoculation and screening for sheath blight in rice.
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However, we found that neutropenic mice develop progressive pneumonia with all pathogens used without recurring to nonphysiologic or traumatic methods for inoculation.
We developed the reproducible method for zoospore production from P. cajani and the method of inoculation for rapid screening for resistance to PB in pigeonpea genotype.
The results indicated that solid-state fermentation (SSF) using barley as the substrate is a suitable method for the inoculation.
We chose this model because the method for bacterial inoculation allows induction of successful infection even in normal mice.
These analyses were used to verify that the described method for biofilm inoculation and formation of each test organism produced equivalent biofilm growth on different rows within and between the MBEC plates over the test period of 30 days.
Methods used for inoculation were previously reported [29], [31], except that inoculum contained 0.1 µg viral RNA extracted from BaMV-S virion derived from BaMV-S infectious clone pCB-infected N. benthamiana [62], [63] or the addition of 0.1 µg satBaMV transcript of pBSF4 [31] or pBSL6 [35].
The design of percutaneous devices, susceptibility of various animal species, bacterial strains, amounts of bacteria, method of inoculation and methods for subsequent evaluation of the infection are discussed in detail.
6) The method of inoculation allows for more than one approach although the original paper utilized gavage and rectal swabbing.
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