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Moreover, in order to further expand the present study and examine the efficacy of Eda-peptide approach as a general method for inhibitor design for any protease including furin, other peptide sequences need to be examined.
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However, a rapid and sensitive TMPK activity assay method suitable for inhibitor screening has been lacking.
In summary, we had established a selective evaluation method for DPP4 inhibitor candidates based on the recombinant human DPP8 and DPP9 proteins.
Analysis of large data sets has led to the recognition of systematic testing problems and development of improvements in methods used for inhibitor measurement.
A statistical experimental design was used to optimise a capillary electrophoretic separation method for eight inhibitors of the angiotensin-converting enzyme: enalapril, lisinopril, quinapril, fosinopril, perindopril, ramipril, benazepril, and cilazapril.
In this study, we have developed generalized method for predicting inhibitors against EGFR not specifically against mutant form of EGFR.
In summary, we have developed a novel NMR screening method for discovering inhibitors against a specific target in cells where a diffusible substrate or product can be monitored.
To achieve this goal, we established a selective evaluation method for DPP4 inhibitors based on recombinant human DPP8 and DPP9 proteins expressed by Rosetta cells.
We had searched the literature and found no classification method for distinguishing EGFR inhibitors from non-inhibitors exist.
A key gap in this docking approach has been the reliance on modeling noncovalent fit between a substrate and an enzyme, using modifications of methods first developed for inhibitor discovery.
An early method for determining ACE inhibitor activity was to measure the rate of hippuric acid production from HHL catalyzed by ACE [26].
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