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Relative mRNA quantification was performed using the ΔCt method for genes with the same amplification efficiency.
To address new challenges in the CTD task, we explore a new entity identification method for genes, chemicals and diseases.
Because we expect the difficulty of the deconvolution problem to increase with the number of transcripts of the gene, we stratify the result by this number: each dot represents the precision and recall of one method for genes with a particular number of transcripts in the UCSC annotation.
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The plasmid and method for gene deletion are shown in Fig. 1a and b, respectively.
Delivering recombinant therapeutic proteins from a universal microencapsulated cell line is an alternate method for gene therapy.
To advance molecular biology in A. aculeatus, we designed and validated a HR method for gene replacement by deleting the Aaku80 gene.
This leads to a rapid and sustained gene expression, thus, physical delivery systems represent a convenient and efficacious method for gene delivery in vivo.
In this paper, we presented a feature selection method for gene data classification that is based on the assessment of discriminatory ability of individual features within a class.
Expression profiles were generated using deep-Serial Analysis of Gene Expression (SAGE) sequencing, a tag-based next-generation sequencing method for gene expression profiling.
The combination of laser microdissection with T7-SAGE is reviewed as a method for gene discovery of candidate molecules that may be essential for early organ morphogenesis.
Successful embryo cryopreservation facilitates the wider application of assisted reproduction technologies and also provides a useful method for gene banking of valuable genetics.
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