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A new analytical method for fatty acid alkyl esters (FAAEs) determination by GC MS in virgin olive oils is proposed.
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A rapid method for fatty acids (FAs) comparative profiling based on carboxyl-specific stable isotope labeling (SIL) and direct infusion electrospray ionization ion mobility mass spectrometry (ESI IM MS) is established.
The subsequent methods for fatty acid methyl ester (FAME) preparation and gas chromatographic (GC) analysis are the same as reported by Wang [ 32].
A few validation analyses were performed to ensure the reliability of the method used for fatty acid analysis in this study; these measures included linearity, precision (repeatability and reproducibility), and recovery tests.
This study is the first report of an automated method for fatty-acid analysis of the phospholipid fraction of human plasma, which includes both phospholipid extraction and FAME derivatization, and has been successfully applied to date to more than 28,000 samples of the InterAct project (detailed information about the subjects can be found in the cohort description [ 17]).
Cells were prepared and equilibrated similar to the methods described above for fatty acid oxidation, with the exclusion of carnitine from the medium.
With the method we have used for fatty acid analysis we did not distinguish between intracellular free fatty acids or those derived from triacylglycerols (TAGs), glycolipids or other lipid classes.
Fluorescence-based methods are advantageous in binding assays, because they were found more suitable for fatty acid binding assays.
For fatty acid analysis by GC-FID, lipid samples were extracted from tissues by the method of Bligh and Dyer.
For some algae, obviously, the method of fatty acid extraction has a significant impact on the final results.
This observation, coupled with the rapid metabolism of yolk-delivered radiolabeled fatty acids (Fig. 3), indicates that yolk injection is an efficient method for delivering fatty acid tracers for metabolic studies.
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