The reasons for discordance between methods for ER and HER2 arise as a result of the different thresholds used for positivity since the cutpoint at which the automated score was dichotomised was not optimised against the manual dichotomous score.
There was a high degree of concordance between Onco type DX and standard IHC methods for ER, PR and HR status, indicating Onco type DX may be a valid alternative to IHC.
Details of the immunostaining are given in Supplementary Methods, along with the scoring methods used for ER, PR, HER2 and BCL2 in the different series.
This current study was designed to evaluate a tissue microarray (TMA -based meTMA -basedssessing ER and PR testing quality.
Although the immediate additional clinical value of this molecular classification is limited by its close correlation to traditional methods of testing for ER and HER2, the identification of genetic aberrations that underlie molecularly distinct subclasses of breast cancer has revealed new therapeutic targets and has reshaped breast cancer clinical trial design.
An illustration of a clear and rational method for deriving an ER to decide whether an individual is an immunological responder or not is given by Dubey et al. [ 14].
The goal of their paper was to advocate a method for developing an ER but explicitly not to recommend the specific cutoff values (x and y) observed in their experiments, as any laboratory would have to identify these themselves, based on their own testing results.
An example of the results obtained using multiple screening methods for one ER-α A908G mutation-positive CBCS tumor is given in Fig. 2. For further assessment of the relative sensitivities of the methods that successfully detected the ER-α mutation, several additional comparisons were made.
To improve the particle preparation method for polyaniline, which generates ER fluids with its semi-conducting characteristics, we synthesized microencapsulated polyaniline particles with melamine formaldehyde (MF) resins.
