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To facilitate data collection, we developed a semi-automated method for embryo imaging and phenotype recording.
As the original invasive method for embryo selection, preimplantation genetic diagnosis (PGD) was initially applied to the screening of X-linked disorders [ 47] and monogenetic diseases with PCR [ 48, 49], and later extended to aneuploidy screening with the use of fluorescence in situ hybridization (FISH) [ 50- 53].
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However, it is now accepted that morphologic parameters are not an entirely reliable index of embryo quality, and, as a consequence, intense interest is focused in developing more reliable methods for embryo selection[11], [12].
Some of these technologies may provide non-invasive methods for embryo evaluation in the future.
Johnson et al. (Johnson et al., 2006) compared several methods for embryo phenotype screening.
In vitro fertilization is the method used for embryo creation, not artificial insemination.
We developed a method for maintaining embryo viability after yolk removal, which allowed for the first time a clear visualization of developing late endocrine cells in living embryos.
With the advantage of the simplicity and efficiency of this technique, LSI can be a proper method for routine embryo's assessment.
Considering the simplicity, cost and the efficiency of this technique, LSI is a proper method for routine embryo's assessment in both research and industry.
More efficient artificial incubation can be achieved using this method for routine embryo's assessment in industry, which provides a potential tool for automatically egg screening during artificial incubation.
Harland, R. M. In situ hybridization: an improved whole-mount method for Xenopus embryos.
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