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In this case, relying on the "adjacent probe" method for deletion detection increases the FPR.
The analysis was performed with the invader method or polymerase chain reaction (PCR -invader method for single nucleotide PCR -invaders (SNPs) of CYP2D6 and UGT1A1 *28 and with the long-PCR method for deletion of CYP2D6 and UGT1A1 gene.
If you're going to this extreme, the method for deletion will depend on whether you're using a paid-for service or a free-roaming service on the web.
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Pindel, an SR approach, performed relatively better than other methods for deletions of 100-10,000 bp.
The plasmid and method for gene deletion are shown in Fig. 1a and b, respectively.
In addition, this method for the deletion of a gene facilitates the production of a revertant control virus in which a full-length gene is reinserted into the same locus [33].
Moon et al.[ 94] also proposed this same ZFN method for SMG deletion in plant pollen.
A dimerizable Cre recombinase (DiCre) technology (Jullien et al., 2003) has been adapted for P. falciparum and the related apicomplexan parasite T. gondii to provide a rapid and efficient method for gene deletion and conditional expression (Andenmatten et al., 2013; Collins et al., 2013).
We have modified the existing method for introducing deletions in the yeast (S. cerevisiae) genome using PCR fragments in order to target point mutations to this genome.
Herein, we report a silver nanoclusters-based (AgNCs-based) fluorescence resonance energy transfer (FRET) method for detection of BRCA1 gene deletion.
In order to assess the reliability of the method for detection of homozygous exonic deletions, we also included a patient with a 298-bp homozygous exonic deletion of STXBP2.
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