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We validate the utility of this method for biochemical and microscopy studies by demonstrating the roles of RhoGTPases in substrate laminin-induced complex cluster assembly.
In this paper we propose a model reduction method for biochemical reaction networks governed by a variety of reversible and irreversible enzyme kinetic rate laws, including reversible Michaelis-Menten and Hill kinetics.
Using the replating method, this time onto coated 100mm culture dishes, we observed that cellular Rac1 is activated within 8h of plating, before the appearance of AChR complex clusters on the adherent muscle cell surface (Fig. 3), demonstrating the utility of this method for biochemical signaling assays associated with AChR aggregation by immobilized ECM components.
Continuous variables were expressed as X ¯ ± s, paired Student's t-test for qRT-PCR data, independent t-test for in situ hybridization result, Kaplan-Meier method for biochemical recurrence free-survival, and Cox regression model for the univariate and multivariate analysis.
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Yet several aspects of self-assembly systems challenge key assumptions of conventional methods for biochemical simulation.
Interrogation of gene regulatory circuits in complex organisms requires precise tools for the selection of individual cell types and robust methods for biochemical profiling of target proteins.
In the future, QDs will be used for identifying various categories of cancer cells, the molecular mechanisms of disease, and new drug action mechanisms, applying them in the intracellular/extracellular studies, and making new methods for biochemical assaying.
Here, we report a novel in planta "exon engineering" strategy for cloning of full-length coding sequence of ABC transporters followed by methods for biochemical characterization of ABC exporters in Xenopus oocytes.
This historical perspective highlights the importance of spectroscopic methods for biochemical studies in general, and especially for heme enzymes, where the presence of the heme iron and porphyrin macrocycle provides rich variety of specific spectroscopic markers available for monitoring chemical transformations and transitions between active intermediates of catalytic cycle.
This transformation will be facilitated by further development and adaptation of analytical and visualization methods for biochemical systems analysis.
Present methods for biochemical and genetic diagnosis are accurate, but depend on clinical suspicion, which could be delayed.
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