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Agar disk diffusion is a traditional and routine method for antimicrobial susceptibility tests [15].
The Disc diffusion method for antimicrobial susceptibility testing was carried out according to a standard method by Bauer et al. [29] to assess the presence of antibacterial activities of the Hap/GA composite.
Mean inhibitory concentrations were performed using a standard method for antimicrobial susceptibility testing in accordance with Twenty-fourth informational supplement M100-S18 (Villanova, PA, USA, 2008).
E-test is a reliable, reproducible, and easily performed method for antimicrobial susceptibility testing and has been successfully employed for the testing of Brucella strains [ 7, 13, 14].
Since the disc diffusion method was found to be a reliable and easy tool for monitoring the prevalence of Campylobacter strains, we applied this method for antimicrobial susceptibility test in this study.
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Their fastidious nature has hampered standardization of methods for antimicrobial susceptibility testing.
The increasing need for novel antimicrobial drugs raises the necessity to establish alternative sources and high-throughput methods for antimicrobial compound discovery.
The MICs for SMT19969, vancomycin, and metronidazole were determined in accordance with National Committee for Clinical Laboratory Standards (2000) methods for antimicrobial susceptibility testing of anaerobic bacteria (12).
The disc preparation was performed following the procedure outlined by the British Society for Antimicrobial Chemotherapy in their Methods for Antimicrobial Susceptibility Testing Version 7, January 2008 [ 15].
The aims of the committee are to standardize methods for antimicrobial sensitivity testing in laboratories throughout the country, collect local data on antimicrobial resistance, and share the information with clinicians and pharmaceutical companies.
However, few isolates from this region have been examined and mostly disc diffusion methods (with lower breakpoints for "less susceptibility" to ceftriaxone and resistance to spectinomycin compared with international MIC-determining resistance methods) for antimicrobial susceptibility testing have been used rather than internationally validated, quality assured MIC-determining methods.
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