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This method first normalizes the decision matrix of a MCDM problem.
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These methods first normalize probe intensities across samples and then look for regions where probe intensities are significantly different between two different groups (e.g. ChIP versus control in a ChIP-chip experiment).
Relative levels of "free" mRNA to "polysome-bound" mRNA were calculated according to the 2 − Δ Δ C T method [ 35], first normalizing the CT of each RNA fraction to the average CT of the presumed unregulated genes (see above), and then setting the amount of "polysome-bound" RNA to 1. Three biological replicates were performed for each gene.
For the ratio system of the MOORA method, first, the decision matrix is normalized using Eq. (2) as seen in Table 4.
Try the natural method first.
Practice the Julienne method first.
The raw signal intensity (CEL file) values were first normalized with MAS5.0 method using affy package in R language (Gautier et al. 2004) and probes were mapped to Locus ids of RGAP.
Because the 3D culture lysates prepared by this method may contain collagen I, we first normalized these lysates for β-actin contents by Western blotting analysis.
The best analysis scheme for our data was to first normalize using the DESeq method and apply a generalized linear model assuming a negative binomial distribution using either edgeR or DESeq software.
We first normalize the microarray data using a rank invariant method.
To predict the class labels of the 6 additional samples, we first normalized and summarized the *.CEL files using RMA method.
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