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The Vu,brain values for all available tracers were determined using a high-throughput brain slice method exactly as described previously [11], employing tracer analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS).
In each sample, the concentration of deuterated cholesterol was normalized to the concentration of native cholesterol, and the area under the curve of the deuterated cholesterol plotted against time was calculated by numerical integration using the trapezoidal method, exactly as described (17).
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All methods were performed exactly as described by Lawson et al[ 24].
Next, biotinylated Hevylite antibody was applied (dilution 1/500 for IgG kappa and 1/800 for IgG lambda) for 75 min. Subsequent washing and detection were performed exactly as in the method above.
The emulsion PCR and subsequent sequencing reaction were performed exactly as described in the GS FLX emPCR Method Manual (USM-00033.A, Roche, Mannheim, Germany).
It is Leonardo's method exactly.
It ran exactly as written.
Timeline works exactly as promised.
Sometimes a novel performs exactly as expected.
Not all worked out exactly as planned.
Instead, it has succeeded exactly as intended.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com