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We expect that the AMT method established here will contribute to functional genetic analyses in A. aculeatus.
While PCT sensitivity detected by the dual-QDs-labeled LFS method established here reached 0.1 ng/mL, which was consistent with the sensitivities detected by immunofluorescence method and immunochemiluminescence.
The method established here would be applicable to other brain regions in many species including those in which development of transgenic animals is technically difficult at present.
The method established here can most likely be applied to a variety of brain regions for studying the function of individual inputs to these regions.
We conclude with the method established here, more modified nucleosides in plants can be investigated in order to understand the function of tRNA modifications in plant growth, development and stress responses.
In contrast to PCR-based detection of RT products or nuclear 2-LTR circles, which has to be performed on bulk extracts, the imaging method established here allows identification of individual RTC/PIC in the physiological environment of the infected cell.
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The method we established here would be useful for analyzing not only retino-tectal vs retino-LGN-V1-sSC pathways, but also other brain regions that receive multiple inputs from different sources.
The screening assay established here has significant advantages over conventional methods, particularly in terms of ease of use, throughput, time and cost.
The direction of causality cannot be established here.
The results to be established here are as follows: (3.1).
The methods we establish here can form the basis for such research.
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