Sentence examples for method essentially as from inspiring English sources

Total RNA was extracted using the hot acid-phenol method essentially as described [15].

Zinc finger arrays were selected using the OPEN method essentially as previously described [26] but with a small number of alterations that improve the speed and throughput of the protocol.

NAA (µmol/L) was measured using a modified stable isotope dilution Gas Chromatography-Mass Spectrometry (GC-MS) method essentially as described by Jakobs et al. [7] In short, 1 nmol [D3]-NAA was added as internal standard to 100 µl of CF. This was also added to aqueous standards.

Drug sensitivity was determined by the MTT method essentially as described by the manufacturer's instructions (Sigma, St . Louis MO, USA).

Na+ and K+ content in plant tissues was determined by the freeze-thaw method essentially as described elsewhere [ 54].

N2aβ and 293T cells were transfected using the calcium phosphate method essentially as described [58].

At least 4 μg of normalised cDNA was submitted to the Advanced Genomics Facility (AGF) at The University of Liverpool where the sample was prepared using methods essentially as described by Margulies et al. [ 31].

These sera were used to test the reactivity of native C. parvum and C. hominis antigenic preparations using Western blotting, according to the standard methods essentially as previously described (Tyler et al. 2009).

These studies employed conventional methods, essentially as described [60], [61].

Pancreatic islets were isolated following standard methods, essentially as described in [32].

The AFLP method was performed essentially as described by Vos et al. [ 16] with minor modifications.