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Sixty polar analytes were first screened to identify those most suitable for inclusion in chromatographic test mixtures; then, a multi-dimensional method development study was conducted to determine the optimal choice of stationary phase, modifier additive and temperature for the separation of such analytes using SFC.
In the present study, we first undertook an extensive method development study of the INNOTEST® Aβ42 ELISA, creating a number of important modifications to the assay.
Calafat et al. (2004), in a method development study, followed the monoester and oxidative metabolites of three pooled U.S. human milk samples and found that most of the oxidative metabolites were at or below the limit of detection (LOD).
This confirms the previous findings from our method development study that found detectable levels of both DBCPP and DPP in all nine samples randomly collected from adults in North America (Cooper et al. 2011).
If a method development study exhibits highly variable results (e.g., high%RSD) or lower than desired percentage recovery values, various parameters should be assessed prior to proceeding to validation including but not limited to the following: (1) container composition; (2) protection from light; (3) temperature; (4) filter bias; (5) dosing apparatus, and (6) stability.
FC: fold change FDR: false discovery rate FPR: false positive rate IQR: inter-quartile range MAS: (Affymetrix) Microarray Suite MM: mis-match (probe) PM: perfect match (probe) qRT-PCR: quantitative reverse-transcription polymerase chain reaction RMA: robust multi-array average ROC: receiver operator characteristic AH participated in all phases of the method development, study design and analysis.
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Publication topics span from chemical and biological detection and quantification method development studies to best management practice studies on the transformation of BPA prior to environmental release (e.g., ozonation and bacterial degradation).
Planning for this large study began in mid-2000 withethe initiation of a number of methods development studies, particularly the development of less burdensome and less costly exposure assessment methods (U.S. EPA 2004a).
HI assisted in the method development and study design.
The use of experimental design in method development was studied for the chiral separation of several amino acid derivatives with capillary electrophoresis.
JH carried out the sample preparations, the detector measurements, the autoradiograms, the statistical calculations and the microscopic investigations; participated in the design and method development of the study and drafted the manuscript.
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