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The extract was sampled using a DVB/CAR/PDMS SPME fibre (50/30 µm) and analysed with the GC MS method detailed above, with extraction time 60 s.
The 14 modelled ligands were built by manually modifying the reference ligand and then energy minimising the ligand whilst keeping the reference heavy atoms fixed according to the method detailed above.
The obtained lysates were subjected to determination of enzymatic activity using the colorimetric method detailed above.
3 Unfortunately, the method detailed above does not consider the effect sensitivity has on image resolution.
We have considered these fused filopodia as one object and have calculated the length of the fused filopodia using the method detailed above.
Turning to bromide capture, the method detailed above proved to be general, and the bromide-containing MAC [{2 R, R -TMCDA}Li]+[Li5(μ-HMDS)5BR -TMCDA}Li]successfully prepared.
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Using the methods detailed above, a total cycle time of 40 minutes was achieved suggesting that pyGC-MS can be utilized for automated studies of 36 samples per day or up to 800 samples per month and instrument, including method blanks and quality control samples.
Removal of low-quality bases and reads and mapping of filtered reads were done following the methods detailed above for single cell and nucleus data analysis.
Within the intervention arm of the study, we compared successful and unsuccessful self managers in terms of the study outcomes, using the methods detailed above.
For each of them we provide a bound on the significance value of a potential optimal solution according to the method detailed in the section above.
While the method detailed in the steps above works by creating air pockets between the pages, this method works because you only tear a few pages at a time.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com