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Although a similar study was recently performed to identify fungal cellulases [ 32], the method described utilized combined secretome and transcriptome analyses and was only aiming to show cellulose activity of the cloned cDNA without the need of the full verified sequences to make all discovered isoenzymes available by recombinant expression.
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The method described here utilizes the proprietary Universal Linkage system (ULS®) technology from Kreatech Biotechnology (Amsterdam, The Netherlands) for linking biotin to amplified RNA [ 2, 3].
In the method described here, we utilized an existing M. jannaschii synthetase-tRNA pair evolved in E. coli, directly in mammalian cells.
The new detection method described above utilizes the unique nature of low-molecular-weight PEI, i.e., it cannot form an insoluble complex with a single-stranded anionic polymer with a low molecular weight such as an oligo DNA probe, but it can form an insoluble complex with DNA with a high molecular weight such as LAMP product.
The method described is charge based, utilizing the difference in the pI between the antibody Fab/F ab′ 2 fragments and antibody Fc fragments that occur after enzyme digestion of whole antibody molecules.
A necropsy based health assessment was performed on all fish using a modification of the method described by [22] and previously utilized to document health of hatchery and wild coho salmon (Oncorhynchus kisutch) [23].
Transformation of S. miltiorrhiza utilized the method described by Horschet et al. [ 33] with modification.
Following completion of the second fluency test, blood pressure and heart rate data were obtained utilizing the method described above.
These systems can benefit from utilizing the method described here to provide additional metabolic data in real-time.
Tactile allodynia was assessed in mice by measuring paw withdrawal thresholds using calibrated von Frey filaments (OptiHair™, Marstock Nervtest™, Germany) utilizing a method described before [ 33].
The matrices of p-values obtained from the point-by-point ANOVAs were corrected for false discovery rate (FDR) utilizing the method described by Benjamini and Yekutieli (2001).
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