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Reverse transcription was performed at 37°C for 60 min with a final denaturation step at 95°C for 5 min. RT-PCR amplification conditions were optimized from the method described from the previous study [ 66].
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The ELISA was adapted from a method described for IgG. 9 Serum or plasma was diluted 1 100.
The method described here differs from previous work in the use of fast fourier transform (FFT) methods to carry out the convolutions.
Concentration of dissolved carbohydrates were directly analysed using the HPLC method described above proceeding from the filtration step.
Our method was modified from a method described previously for isolation of EcO157 from cattle feces, carcass and hide samples [44].
Vermiwash was extracted by three different methods which are as follows: This method was adopted from method described by Karuna et al. (1999).
The method described here was adopted from a published procedure of O'Brien for mice and rats (16).
THg and IHg were then measured by the analytical method described above, and IHg from demethylation of MeHg was calculated as the percentage of the initial amounts.
The procedure for total RNA isolation from latex was derived from the method described by Kush et al. [ 83].
Porphyrins were extracted from liver using the method described by De Matteis and Lim [ 20] and from faeces according to the method of Lockwood et al. [ 21].
For liver, brain, and whole-pup samples, extraction procedures were derived from Folch et al. (1951) and from the method described by Waters et al. (2002).
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