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HPLC-UV method described at the 'Quantitative method for atrazine' section was utilized to quantitate the concentrations of adsorbate.
NFAP2 was purified from this mycelia-free supernatant based on the slightly modified method described at NFAP previously (Virágh et al. 2014).
The testing method described at the beginning of the section showed that all 238 reaction templates resulted in the correct products being formed.
Owing to animal age (3 months old at the time of dissection), we used the 'protective recovery method' described at http://www.brainslicemethods.com.
Transgene genomic location was assessed by inverse PCR accordingly to the method described at http://www.fruitfly.org/about/methods/inverse.pcr.html (Berkeley Drosophila Genome Project).
The alternate method described at that time was read alignment with GSNAP and variant detection with GATK and best practices, providing a sensitivity of 96 97 %, but it incurred at least 8 additional hours of computation (total time 58 h) [ 12].
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Using purified MPZ-ZAI and VACV-WR virus genomic DNA as a template, PCR reactions were performed and PCR products were purified (according to the methods described at (http://www.microarray.org/doc/protocol/PCRprotocol.rtf) and each product was transferred twice to a 384-well plate (http://www.microarray.org/doc/protocol/96_to_384_well_transfer.rtf).org/doc/protocol/96_to_384_well_transfer.rtf
The PUTs sets were generated by assembling 128 098 ESTs and mRNA sequences (version 175a) using the methods described at the site (http://www.plantgdb.org/prj/ESTCluster/PUT_procedure.php).php
We followed the methods described at the following web address: http://www.mdanderson.org/education-and-research/resources-for-professionals/scientific-resources/core-facilities-and-services/functional-proteomics-rppa-core/index.html.html
For evaluation of potential demethylation during solubilisation, a purified (> 99%) radiolabeled methylmercury-chloride (Hg, Amersham Laboratories, Amersham, UK) solution was solubilised according to the method described above, at room temperature and at 88°C for one hour.
Quality control (QC) samples were prepared following the same sample preparation method described above at the concentrations of 20, 1000 and 10000 ng mL−1, respectively.
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