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Biosurfactant production ability of these different strains was evaluated through oil spreading method (data not shown).
We analyzed the insertion position by the genome walking method (data not shown).
Furthermore, the fusion genes of rhabdomyosarcoma (PAX3 FKHR and PAX7 FKHR) were not detected on polymerase chain reaction-based method (data not shown).
Secreted TNF-α protein could not be detected by the same method (data not shown).
Using phosphorylated TCs, we observed a greatly enhanced efficiency of the method (data not shown).
Otherwise, there was no sequence homology or structural resemblance as indicated by the HCA method (data not shown).
Notably, 5D9 did not exhibit significant cytotoxicity at these concentrations in parallel assays using the XTT method (data not shown).
Results were in agreement with the data obtained by the tryptan blue exclusion method (data not shown).
The diversity of subtypes in G2 was confirmed by phylogenetic analysis by Neighbor Joining method (data not shown) and Maximum Likelihood inference (Figure 2).
At these concentrations punicic acid did not affect PMN viability as determined by the trypan blue exclusion method (data not shown).
Despite this degree of disagreement, estimates of heterozygosity do not change appreciably when using SNP information from the capillary sequencing method (data not shown).
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