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A fast gradient HPLC method (cycle time 15 min) has been developed to determine Human Serum Albumin HSAA) binding of discovery compounds using chemically bonded protein stationary phases.
With this method, cycle ambiguity can be resolved with less stringent conditions in which the phase error of the residual phase is less than 0.2 rad.
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If this is correct, he writes, then "millions of rhythm method cycles per year globally depend for their success on massive embryonic death".
We used the following PCR cycling protocol: an initial denaturation step of 7 min at 96 °C followed by 20 touch-down method cycles of 45 s at 96 °C, 45 s at 62 °C → 52 °C, 60 s at 72 °C, followed by 15 cycles of 45 s at 96 °C, 45 s at 52 °C, 60 s at 72 °C and the final extension period of 10 min at 72 °C.
We used the following PCR cycling protocol: an initial denaturation step of 5 min at 94 °C followed by 20 touch-up method cycles of 60 s at 94 °C, 90 s at 44°→ °C °C, 1 min at 72 °C, followed by 15 cycles of 90 s at 94 °C, 90 s at 53.5 °C, 60 s at 72 °C and the final extension period of 7 min at 72 °C.
In their methods, cycle ambiguity was derived analytically.
Capacitance specifications were calculated by these methods: cycle voltammetry and galvanostatic charge/discharge [7, 12, 13].
To ensure valid and high-quality methods, cycle ergometer testing and direct continuous measurement of respiratory gas exchange will be used to estimate the interventional effects on CRF.
In our method, one cycle with average expression value at every time point of three successive cycles is used to reduce the noise and error.
It affects the selection of excavation method, excavation cycle and rock reinforcement method and time to install rock support.
The polynomial fitting method for cycle slip detection/validation is not new.
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