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Quantification was carried out using a fundamental parameters method corrected with the use of an international standard (USGS W-2a) [7].
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The Ct values obtained were converted to relative expression values by the comparative Ct method, correcting with the values obtained for actin and normalizing to the untreated control levels.
Melting points were determined by open capillary method and are corrected with standard benzoic acid.
Affymetrix Human JAY arrays were normalized using the probe scaling method and background corrected with ProbeEffect from GeneBase (Kapur et al., 2008).
Data analysis was done using the comparative CT method and data were corrected with the GAPDH mRNA levels.
With this method, vision can be corrected with a better quality of vision regarding contrast sensitivity.
In this method, the emission sinogram is preliminarily corrected with the standard corrections, then convolved with the kernel, and the tails of the convolved sinogram and the measured sinogram are fitted to obtain a scaling factor for the convolution kernel.
P values were obtained using paired two-tailed Wilcoxon rank-sum tests and corrected with Benjamini Hochberg method and are indicated above each graph.
Pairwise comparisons of automation level were corrected with Tukeys method when sphericity was assumed and otherwise with Bonferroni.
Other common problems, such as communication faults, could also have easily been detected and corrected with our method.
Electrostatic interactions were corrected with PME method [ 59], using non-bonded cutoffs of 1.0 nm for Coulomb and 1.2 nm for van der Waals.
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