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The novel method contained an additional six-hour bioprocess and a six-hour lixiviant leaching.
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The genome obtained using this method contained a single 2.72 Mb chromosome without gaps.
The libraries produced by this method contained a very high proportion of full-length cDNAs and produced an excellent yield without involving PCR amplification which could introduce bias in the representativeness of the clones.
The authors themselves highlighted that this method contain an important assumption: the temperature distribution on the receiver surface is independent of the incident power level.
The method contains an eddy-resolving circulation model, a scheme for tracking of Lagrangian trajectories, a technique for the calculation of quantities characterising the potential of different sea areas to supply adverse impacts, and routines to construct the optimum fairway.
Normally, this method contains an intrinsic sorting mechanism, in which excessively large particles settle by static placement, and the supernatant, containing finer nano-sized particles as the dispersion, simply collected.
It is estimated internally, during the run as the RF method contains an inherent cross-validation procedure, which is used to identify the relative importance of each independent variable.
The method contains an initial phase of preparation and organisation, including open coding, category formation and abstraction [ 36- 38].
Furthermore, the method contains an internal positive control (the shared primer), but should always be tested in samples of known sex before actual analysis is carried out.
This first method contains an inherent bias in favor of AT-biased conversion because of the greater genetic distance between DT and D5 compared to the distance between AT and A1 or A2.
These results indicate that sericin extracted from the mutant cocoons via the LiBr method contains a putatively intact protein profile.
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