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Based on the results achieved upon the method comparison conducted using a Ag ENP suspension, ultrafiltration was identified as best practice for the determination of the dissolved fraction of the other ENP suspensions used within the comparison of the sample preparation procedures.
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For the two-method comparisons conducted (sample preparation procedures and determination of the dissolved fraction), the stock suspensions were diluted to working suspensions (WS) of a mass concentration of approximately 1 to 10 mg/L (refer to the Electronic Supplementary Material, Table S 1).
Nevertheless, the totality of these genes was also detected by the SAM Method in the three comparisons conducted.
Using average DNA methylation assessed by the array, samples clustered most strongly by placental vs. somatic origin (Fig. S1 ), and then by tissue type; thus, inter-method comparisons were conducted within each somatic tissue (fetal brain, kidney and muscle and adult blood) and then collectively in the merged somatic group.
The validity of a MTC depends on the method: were unadjusted or adjusted indirect comparisons conducted?
Consider a measurement method comparison study that is conducted using m = 1,..., M methods, M ≥ 2 on i = 1,..., N subjects and that for each method and subject r = 1,..., R mi repeated measurements are made.
All of the comparison methods were conducted using their released codes, and the data had been tuned to better adapt for our experiments.
Method comparisons were made between GIA conducted with the same parasite line using the preferred methodology of the testing laboratory (summarized in Table 1).
Fig. 4 Method comparison.
Since robustness is an important feature of a method, we conducted six comparisons to examine the robustness of the methods.
The Duncan test method was conducted for multiple comparisons to assess the significance level of each parameter among treatments when the main effect was significant.
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