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The classification is based on Ward's method, checked by discriminant analyses.
The precision of the method, checked by repetitive analyses of the same sera, showed an average coefficient of variation of 5.7%.
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The validity of this method was checked by a more direct method, in which molecules were labeled either as plus or minus at the beginning of a simulation, and the labels were complementarily copied when replication happens.
Second, the accuracy of this method is checked by using the Rayleigh-Ritz method, which removes the restrictive hypothesis on the wet mode shapes.
The validity of the proposed method was checked by applying the standard addition method and the recoveries at the 20 μg L−1 level using both inorganic and organic metal standards ranged from 95%to99%95%to99%
The inter-rater reliability of the Angoff method was checked by using Intra Class Correlation Coefficient (ICC) [ 20] employing the average method of reliability and two-way random effects model.
The convergence of this method was checked by error calculation.
The proposed method is checked by numerical simulation.
The accuracy of the method was checked by the standard addition technique.
Precision and accuracy of the developed method was checked by recovery studies.
This "cubication" method is checked by studying the period, energy and solution for several examples.
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