Direct transformation methods such as particle bombardment can frequently result in the insertion of large copy numbers of plasmid DNA at a single-site, leading to transgene silencing [ 63, 64].
Although essentially any reproducible digestion method can be used, MRM assays most frequently are based on tryptic peptides, which typically range from 5 to 25 amino acids in length and usually form multiply charged positive ions, which provide useful sequence information through MS/MS fragmentation.
By exploring keywords, which frequently co-occur, as patterns, our method can maximally preserve context information in the topic mining process.
Thus, our CNF method can sample native-like angles at these residues more frequently than the CRF method and avoid generating a large number of mirror images.
Cytological assessment is currently the frontline method for identifying precancerous cervical lesions however repeat evaluations can frequently be required due to low sensitivity [ 3].
Nonetheless, predictions of a deleterious effect should be taken with caution as neutral polymorphisms can frequently be misclassified (from ~15-50 ~15-50ding on the method [ 28]).
The use of FSVPET can therefore only be recommended for tracers which frequently require dynamic scanning and absolute quantification, as the method can be incorporated in the standard analysis only in these cases.
Finally, the hybrid method can be used as an alternative to adaptive mesh refinement to resolve boundary layers that are frequently present around a stiff immersed interface.
The Socratic Method can be quite intimidating.
Goblins can frequently be found with bows.
They can frequently be used in place of essential oils when dilution is not an issue, as in some aromatherapy methods.
