However, due to high sequence homology among 16S rRNA genes and consequently, very few specific regions, we developed a novel NASBA method called "single specific primer-NASBA or SSP-NASBA" in which the specificity of the antisense primer is sufficient to perform a specific NASBA reaction.
This method, called SPARE (Specific Parallel Amplification of 5′ RNA Ends) allows the identification of processing intermediates for most of the Arabidopsis miRNAs.
The network structure was designed by a specific method, called leave-one-out cross-validation.
In the case of the large oceanic basins, a specific method, called the bathymetry optimisation method, is needed to correctly take into account the bottom topography inside the model.
The second method, called CRISPR-Cas, cleaves specific DNA sequences that have been targeted by designer RNA sequences (Haurwitz et al., 2010).
Both the detection method and the reference set of genomes to be blasted are important for identifying lineage-specific genes, so we used a method called 'phylostratigraphy' to obtain the lineage-specific genes within zebrafish [ 32, 61].
To further increase throughput, target specificity, and cost effectiveness we developed a novel genotyping method called MIT-NGS that uses highly specific primer and PCR conditions to amplify full-length HLA-A, B, C and DRB1 loci from 96 individuals.
Using a novel method called SWITCHplus, we identified subtype-specific DNA CNAs occurring at a 15%% or greater frequency, which excluded many well-known breast cancer-related drivers such as amplification of ERBB2, and deletions of TP53 and RB1.
A British clinical hypnotherapist, Michael Mahoney, has developed an IBS-specific self-hypnosis method called the "IBS Audio Program 100", to be practised by IBS patients in their own time, at home.
A specific formulation of the proposed method, called hybrid regularization, is introduced and investigated.
