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qPCR was modified from our previously established method by targeting DEFB4[ 17].
On the other hand, BrRNA-RT-qPCR may represent a more sensitive method by targeting ribosomal RNA.
In effect, this feature enhances the reproducibility of the restriction-based method by targeting fragments of a given size.
Large-scale functional gene microarrays such as the Geochip has been chosen as an alternative method by targeting several specific genes involved in ecosystem functioning.
Throat swab specimens and sputum samples were collected and analyzed by using real-time reverse transcription PCR (RT-PCR) performed on the basis of a previously reported method by targeting the upstream E region and open reading frame 1b of the virus (5 ).
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In Phase 1, we piloted the methods by targeting all children from 5 to 12 years registered in the public school system of a city (Santa Cruz das Palmeiras, São Paulo, Brazil).
We evaluate the performance and the limits of photogrammetric methods by targeting a set of fundamental parameters necessary to study biogeomorphic feedbacks at each of the three nested spatial scales and, when possible, propose appropriate solutions.
The method was demonstrated by targeting five clinically meaningful mutations in MDRTB.
The method was evaluated by targeting three different rice genes, Phytoene desaturase (Pds, Os03g08570), Spotted leaf 11 (Spl11, Os12g38210), and Eui1 (CYP714D1, Os05g40384) (Table 1).
The first method is performed by targeting particular candidate genes or by quantitative trait loci (QTL) mapping and positional cloning of QTL.
We demonstrated the efficiency and specificity of this method in planarians by targeting and measuring the protein products of the myosin and tubulin genes, which appeared in press a year later [ 23].
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