Sentence examples for method by normalizing from inspiring English sources
Quantitative Real-time PCR was performed in a StepOne Plus Real-Time PCR System using TaqMan gene expression assays for VCAM-1 and GAPDH with TaqMan Gene Expression Mastermix (Applied Biosystems, Warrington, UK) Data were generated by the comparative threshold cycle (ΔΔCT) method by normalizing to Glyceraldehyde 3-phosphate dehydrogenase (GAPDH).
Samples were compared using the ΔΔCT method by normalizing to SnoRNA202 expression and the median array.
Relative expression was calculated using the ΔΔCt method by normalizing to the house keeping genes ACTB and GAPDH.
Each sample was assayed in duplicates and the relative gene expression was quantified using ΔΔCT method by normalizing with β-2 myoglobulin as a housekeeping gene.
The relative amount of target mRNA was determined using the comparative threshold (Ct) method by normalizing target mRNA Ct values to those for β-Actin (Δ Ct).
Expression levels for mRNA transcript were determined by the 2-ΔΔCT method by normalizing each group to the uninjured muscle of the SED group [ 5].
Relative expression of ISGs was determined using the 2−ΔΔCT method [15], by normalizing to GAPDH expression levels.
For quantification, data were analyzed by the 2-DDCT method [ 71], by normalizing to the housekeeping gene rpl7 (ribosomal protein l7).
Relative expression was calculated by the ΔCT method [ 66] by normalizing the CT levels of target genes to the geometric mean of CT levels of two housekeeping genes, cytoplasmic ribosomal protein L2 and elongation factor 1-α(EF1-α). Specificity of amplification was assessed by dissociation curve analysis and running the PCR product on gel.
We modified these methods by further by normalizing FXN to mitochondrial cytochrome oxidase expression, i.e. FXN/Complex IV (CIV).
Ribosome density profiles for well-expressed genes (i.e., with reliable ribosome profiling data, 'Materials and methods') were calculated by normalizing the ribosome density in codons to the median density of the gene.
