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Crystals were successfully grown at 18°C using the sitting drop vapor diffusion method by mixing equal volumes of protein and reservoir solution.
Crystals were grown at 18°C using the hanging-drop vapor diffusion method by mixing 1.0 μL protein solution with 1.0 μL reservoir solution and equilibrating against 200 μL reservoir solution.
In this study, the authors report the production of nanocomposite-enhanced phase-change materials (NEPCMs) using the direct-synthesis method by mixing paraffin with alumina (Al2O3), titania (TiO2), silica (SiO2), and zinc oxide (ZnO) as the experimental samples.
The final purified P protein was concentrated to 10 mg/mL and crystallized with hanging drop vapor diffusion method by mixing equal volume of P protein with reservoir solution containing 0.1 mol/L LiCl, 18% (w/v) PEG 3350 and 10% (v/v) 2-Methyl-2,4-Pentanediol (MPD) at 16°C.
Specifically, iron oxide nanocrystals were formed via the alkaline precipitation method, by mixing a solution of iron salts (0.202 g FeCl2.4H2O, 0.488 g FeCl3.6H2O, 88.7 µL HCl in 2 mL distilled water) with an ammonium hydroxide solution (830 µl NH4OH in 15 mL distilled water).
Needle-shape crystals of eIF3b-RRM76 170 were obtained at 20°C using sitting drop method by mixing equal volumes of protein (11 mg.mL−1) with 30% PEG 4000, 200 mM Li2SO4 and 100 mM Hepes pH 8. Crystals of good diffraction quality grew after three days to the final size of 100*20*10 micrometer.
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VOx/MgO aerogel catalysts were synthesized using three different preparation methods: by mixing the aerogel MgO support with dry ammonium vanadate, by vanadium deposition from a precursor solution in toluene, and by hydrolysis of a mixture of vanadium and magnesium alkoxides followed by co-gelation and supercritical drying.
Crystals suitable for structure determination were obtained using microbatch methods by mixing equal volumes (2 μl) of 15 mg/ml RII tetra-tandemer with the same precipitant solution as above, followed by the addition of 0.5 μl of 5% (w/v) n-Octyl-β D-glucoside.
siRNA/C1A and siRNA/C8A complexes were prepared as described in Materials and Methods by mixing siRNA in nuclease-free H2O with the appropriate volumes of C1A or C8A solution (1.0 mg/mL), such that C/P ratios (ratio of cationic group in the glycopolymer to phosphate group in DNA) were 1.3.
As pointed out by Boutonnet et al., there are two main ways of preparing nanoparticles from the microemulsion method: (1) by mixing two microemulsions, one containing the precursor and the other, the precipitating agent; and (2) by adding the precipitating agent directly to the microemulsion containing the metal precursor [26].
Briefly, cationic phospholipid liposomes consisting of 90 wt % hydrogenated l-α-phosphatidylcholine (EggPC) and 10 wt % 1,2-di- 9 Z-octadecenoyl)-3-trimethylammonium propane (DOTAP) were prepared through the standard extrusion method, followed by mixing with AuC at a liposome-to-AuC molar ratio of 1,2-di- 9
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