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We modified the classical calcium phosphate transfection method by detaching adherent chondrocytes with trypsin and resuspending in CaPo4 nucleic acid precipitate followed by readherence for 24 h.
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Resection was completed by detaching the tumor from the diaphragm.
By detaching themselves from work, monastics cannot place labor ahead of prayer.
Detox by detaching from your e-dictions.
Cells were passaged by detaching through scraping in HBSS.
When cell confluence was reached, cells were subcultured by detaching with 0.05% trypsin-EDTA and reseeding.
The day of passaging, EBs were initiated by detaching cells with Accutase and gentle cell scraping.
It should disconnect by detaching the clip.
A method for detaching the biofilm is required to either enumerate or characterize these bacterial communities.
We present here an improved EB/AO staining method by substituting the detaching and washing steps with a simpler centrifugation step with 96-well plates.
Flow past a circular cylinder at two subcritical Reynolds numbers (Re=3900 and Re=1.4×105) is simulated by partially-averaged Navier Stokes (PANS) method and detached eddy simulation (DES).
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