Some known oncogenes and suppressors in GBM were not detected by the entropy method, but were correctly identified by the combined GISTIC and GTS analysis (prevalence in parentheses): MET (3%), CDK6 (1%), TP53 (1%), CCND2 (2%) and PIK3CA (2%).
These species were not accurately identified by the conventional method but were identified by the present PCR- and sequencing-based method.
These households were also selected with a stratified, multi-stage clustered sampling method but were based on 2009 National Resident demographics.
An additional seven GNGs corresponding to blocked reactions were fixed by this method but were not added to the list of proposed model modifications due a lack of experimental validation (see Additional file 2 for uncorroborated model changes).
To further validate the hypothesis of an alternative promoter, we first tried a direct approach using a 5′-RACE PCR method but were unsuccessful in obtaining products starting before exon 3, probably owing to the presence of a tertiary structure interfering with the PCR-elongation process.
Only two of the five positive ESβL real-time PCR samples were identified by the phenotypic method as ESβL producers; the other two were identified as ESβL producers by the phenotypic method but were negative by real-time PCR (Table 1).
