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This is most probably caused by the interpolation artifacts induced by the DC method as these get stronger the farther away a patch is from the CoD.
The curriculum-based measures are a unique method as these are sensitive to and are validated to monitor progress compared to curriculum-based assessments.
Secondly, although we examined at least 2 samples per patient, we used only a qualitative concentration/sedimentation method as these screenings were not prejudiced towards the detection of Fasciola eggs alone.
However, a study interested in comparing CMD internationally should consider utilising the Youden Index or the (0,1) method, as these methods are most appropriate when the study area encompasses regions with different case prevalences.
It seems likely that that the sheer number of reads from genes of this category would affect quantification using our frequency-weighted method as these proteins are among the most commonly encountered in metagenomics data sets.
For example, the generation of unstabilized diazo compounds in flow has proven to be an attractive method, as these reactive intermediates can be combined directly with a variety of substrates under mild reaction conditions.[ 3a, b] Allenes are interesting functional groups owing to their orthogonal π bonds.
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Both the strong points of these methods, as well as their weak areas, have been highlighted.
An overview of these methods, as well as related references, can be found in [1],[2].
Some local detail in the background seems to be enhanced by these methods as well.
These methods as well as visual assessment of the raw HRV data are the most common approaches used in clinical practice.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com