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Stoichiometry counting was performed using the brightness analysis method as previously described.
Proteins were isolated using the phenol extraction method as previously described104.
Relative expression value was normalized to RPL13a and calculated by using the 2- ΔΔCt) method as previously described.
The qualitative screening was performed by an adapted disc diffusion method as previously reported [24].
A surfactant-assisted solvothermal method, as previously reported by Agiral et al. [37].
SWCNT were dispersed by acetone/sonication method as previously described [10].
Glycogen content was determined using an amyloglucosidase digestion method as previously described [18].
CBF was calculated using the microsphere method, as previously described [3], with slight modification.
Cell viability assay was performed by the MTS method as previously described.
Fifteen formulations of Doc-loaded NCs were prepared by emulsion-diffusion method as previously described [19].
Creatinine was measured using a modified Jaffe method as previously described (Roche Diagnostics, IN, USA) [31].
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