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The method, as originally proposed, did not use TOF information and, therefore, the algorithm had a non-unique solution.
This is for instance done by simultaneous reconstruction of attenuation and activity with the MLAA algorithm, but the method as originally proposed has certain limits.
This method, as originally published, cannot resolve causality, i.e. which gene is the regulator and which gene is the target for a given significant interaction, as it relies solely on the symmetric MI matrix and can not take advantage of the kinetics represented in time series data.
Isolation of total RNA was performed by the guanidium isothiocyanate method as originally described by Chomczynski and Sacchi [ 16].
*HLM, historical limits method; HLMoriginal, method as originally applied in New York City before May 20 , 2013 HLMrefined, refined method applied starting May 20 , 2013
*HLM, historical limits method; HLMoriginal, method as originally applied in NYC prior to May 20 , 2013 HLMrefined, refined method applied starting May 20 , 2013 UHF, United Hospital Fund.
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The multimodal and symmetric calculi of §3.1 and §3.3 pose a challenge to the proof net methods as originally developed for linear logic.
Furthermore, the present study aimed to examine PDE methods as originally presented, without attempting to improve performance through optimizing procedures.
That is, we show that NetDis does not outperform the other methods, as originally claimed, while it is also computationally more expensive.
Furthermore, the numerical multicomponent geothermometry method as proposed originally by Reed and Spycher (1984) is assessed, evaluating if its statistical nature can overcome dependence upon reservoir rock composition.
Malondialdehyde (MDA) was measured based on thiobarbituric acid reacting substances (TBARS) method as described originally 28 with some minor modifications.
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